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Genisphere llc 3dna array 900 kit
3dna Array 900 Kit, supplied by Genisphere llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3dna+array+900/3dna+array+350+kit/pmc02874553-256-14-18
Average 90 stars, based on 1 article reviews
3dna array 900 kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Hybridization:

Article Title: Transition from parenteral to enteral nutrition induces immediate diet-dependent gut histological and immunological responses in preterm neonates.
Article Snippet: Jayda Siggers, Per T. Sangild, Tim K. Jensen, Richard H. Siggers, Kerstin Skovgaard, Ann Cathrine F. Støy, Bent B. Jensen, Thomas Thymann, Stine B. Bering, and Mette Boye Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Frederiksberg; National Veterinary Institute, Technical University of Denmark, Denmark; and Department of Animal Health and Bioscience, Aarhus University, Tjele, Denmark

Article Title: A comprehensive transcriptome analysis of silique development and dehiscence in Arabidopsis and Brassica integrating genotypic, interspecies and developmental comparisons
Article Snippet: Initial data extraction Calculation of the signal intensity of spots and flagging of bad spots were carried out in GenePix Pro 6.1 software (Molecular Devices). .. Five micrograms of total RNA isolated from whole siliques of Arabidopsis and manually dissected valves of Brassica were used for cDNA synthesis using 3DNA Array 900 (Genisphere Inc.. cat# W500130 and W500140) and at least 6 independent hybridizations were performed for each type of sample. cDNA was labeled either with Cy3 or Cy5 followed by hybridization onto full genome Arabidopsis oligonucleotide microarray slides obtained from the University of Arizona (http://ag.arizona.edu/microarray/) and washed according to the manufacturer's instructions for 2 color hybridizations except for prehybridization. ..

Incubation:

Article Title: Transition from parenteral to enteral nutrition induces immediate diet-dependent gut histological and immunological responses in preterm neonates.
Article Snippet: Jayda Siggers, Per T. Sangild, Tim K. Jensen, Richard H. Siggers, Kerstin Skovgaard, Ann Cathrine F. Støy, Bent B. Jensen, Thomas Thymann, Stine B. Bering, and Mette Boye Department of Human Nutrition, Faculty of Life Sciences, University of Copenhagen, Frederiksberg; National Veterinary Institute, Technical University of Denmark, Denmark; and Department of Animal Health and Bioscience, Aarhus University, Tjele, Denmark

Labeling:

Article Title: Virulence Gene Regulation by CvfA, a Putative RNase: the CvfA-Enolase Complex in Streptococcus pyogenes Links Nutritional Stress, Growth-Phase Control, and Virulence Gene Expression
Article Snippet: .. Synthesis and labeling of cDNA used a commercial kit (3DNA Array 900; Genisphere), and the signal of cyanine 3 (Cy3) or cyanine 5 (Cy5) was measured using a laser scanner (ScanArray Express HT; Perkin-Elmer). ..

Article Title: Virulence Gene Regulation by CvfA, a Putative RNase: the CvfA-Enolase Complex in Streptococcus pyogenes Links Nutritional Stress, Growth-Phase Control, and Virulence Gene Expression
Article Snippet: .. Synthesis and labeling of cDNA used a commercial kit (3DNA Array 900; Genisphere), and the signal of cyanine 3 (Cy3) or cyanine 5 (Cy5) was measured using a laser scanner (ScanArray Express HT; Perkin-Elmer). ..

Article Title: A comprehensive transcriptome analysis of silique development and dehiscence in Arabidopsis and Brassica integrating genotypic, interspecies and developmental comparisons
Article Snippet: Initial data extraction Calculation of the signal intensity of spots and flagging of bad spots were carried out in GenePix Pro 6.1 software (Molecular Devices). .. Five micrograms of total RNA isolated from whole siliques of Arabidopsis and manually dissected valves of Brassica were used for cDNA synthesis using 3DNA Array 900 (Genisphere Inc.. cat# W500130 and W500140) and at least 6 independent hybridizations were performed for each type of sample. cDNA was labeled either with Cy3 or Cy5 followed by hybridization onto full genome Arabidopsis oligonucleotide microarray slides obtained from the University of Arizona (http://ag.arizona.edu/microarray/) and washed according to the manufacturer's instructions for 2 color hybridizations except for prehybridization. ..

other:

Article Title: Hepatic gene expression changes in pigs experimentally infected with the lung pathogen Actinobacillus pleuropneumoniae as analysed with an innate immunity focused microarray.
Article Snippet: Briefly, the two cDNA preparations (infected vs control) 25 ml each, 1 ml salmon sperm DNA (10 mg/ml) and 51 ml 2 formamidebased hybridisation buffer (3DNA Array 900, Genisphere) were mixed and 70 ml of this solution was applied to the microarray slide under a 25 60 mm LifterSlip (Erie Scientific Company, Portsmouth, NH, USA) carefully avoiding air bubbles.

Article Title: Gene profiling for studying the mechanism of aflatoxin biosynthesis in Aspergillus flavus and A. parasiticus.
Article Snippet: Aflatoxins are toxic and carcinogenic polyketide metabolites produced by certain fungal species, including Aspergillus flavus and A. parasiticus.. Many internal and external factors, such as nutrition and environment affect aflatoxin biosynthesis; therefore, we analyzed the transcriptome of A. flavus using expressed sequence tags (ESTs) from a normalized cDNA expression library constructed from mycelia harvested under several conditions.. A total of 7218 unique ESTs were identified from 26 110 sequenced cDNA clones.

Generated:

Article Title: Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity.
Article Snippet: Total RNA was lyophilized under vacuum for transport to the Washington University Genome Sequencing Center where RNA quality was verified using anAgilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. First-strand cDNAwas generated by oligo-dT primed reverse transcription (Superscript II; Invitrogen) using the 3DNA Array 900 kit (Genisphere, Hatfield, PA USA). ..

Reverse Transcription:

Article Title: Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity.
Article Snippet: Total RNA was lyophilized under vacuum for transport to the Washington University Genome Sequencing Center where RNA quality was verified using anAgilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). .. First-strand cDNAwas generated by oligo-dT primed reverse transcription (Superscript II; Invitrogen) using the 3DNA Array 900 kit (Genisphere, Hatfield, PA USA). ..

Isolation:

Article Title: A comprehensive transcriptome analysis of silique development and dehiscence in Arabidopsis and Brassica integrating genotypic, interspecies and developmental comparisons
Article Snippet: Initial data extraction Calculation of the signal intensity of spots and flagging of bad spots were carried out in GenePix Pro 6.1 software (Molecular Devices). .. Five micrograms of total RNA isolated from whole siliques of Arabidopsis and manually dissected valves of Brassica were used for cDNA synthesis using 3DNA Array 900 (Genisphere Inc.. cat# W500130 and W500140) and at least 6 independent hybridizations were performed for each type of sample. cDNA was labeled either with Cy3 or Cy5 followed by hybridization onto full genome Arabidopsis oligonucleotide microarray slides obtained from the University of Arizona (http://ag.arizona.edu/microarray/) and washed according to the manufacturer's instructions for 2 color hybridizations except for prehybridization. ..

cDNA Synthesis:

Article Title: A comprehensive transcriptome analysis of silique development and dehiscence in Arabidopsis and Brassica integrating genotypic, interspecies and developmental comparisons
Article Snippet: Initial data extraction Calculation of the signal intensity of spots and flagging of bad spots were carried out in GenePix Pro 6.1 software (Molecular Devices). .. Five micrograms of total RNA isolated from whole siliques of Arabidopsis and manually dissected valves of Brassica were used for cDNA synthesis using 3DNA Array 900 (Genisphere Inc.. cat# W500130 and W500140) and at least 6 independent hybridizations were performed for each type of sample. cDNA was labeled either with Cy3 or Cy5 followed by hybridization onto full genome Arabidopsis oligonucleotide microarray slides obtained from the University of Arizona (http://ag.arizona.edu/microarray/) and washed according to the manufacturer's instructions for 2 color hybridizations except for prehybridization. ..

Microarray:

Article Title: A comprehensive transcriptome analysis of silique development and dehiscence in Arabidopsis and Brassica integrating genotypic, interspecies and developmental comparisons
Article Snippet: Initial data extraction Calculation of the signal intensity of spots and flagging of bad spots were carried out in GenePix Pro 6.1 software (Molecular Devices). .. Five micrograms of total RNA isolated from whole siliques of Arabidopsis and manually dissected valves of Brassica were used for cDNA synthesis using 3DNA Array 900 (Genisphere Inc.. cat# W500130 and W500140) and at least 6 independent hybridizations were performed for each type of sample. cDNA was labeled either with Cy3 or Cy5 followed by hybridization onto full genome Arabidopsis oligonucleotide microarray slides obtained from the University of Arizona (http://ag.arizona.edu/microarray/) and washed according to the manufacturer's instructions for 2 color hybridizations except for prehybridization. ..



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